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Main Procedures Review When reconstituting the compound, use sterile water and swirl gently avoid vigorous shaking, as it can compromise the compounds stability

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More examples of HS-AFM imaging studies on proteins and cells can be found in a comprehensive review by Toshio Ando, Takayuki Uchihashi, and Simon Scheuring ( Although HS-AFM was invented after the IDP field was established, this technique provides unique structural and dynamic information at the single molecule level, generating actual movies showing how IDP moves and directly observing individual protein molecules in action at high spatiotemporal resolution (Miyagi et al., 2008)