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This may lead to: Brain fog Anxiety and irritability Mental fatigue Poor memory and focus Trouble sleeping One study showed that people with relatively more glutamine than glutamate in the brains motivation center had better stamina, less fatigue, and lower perceived effort during demanding tasks 8

The reason is we are addicted to caffeinated beverages, but we must understand the consequences of their daily consumption

BMC bioinformatics 9, 529, doi: 10.1186/1471-2105-9-529 (2008)

PMID 11687296

This matters because when those signals are overly active or out of sync, they may be associated with a wide range of symptoms, and researchers want to determine whether LDN affects that signaling in a measurable way