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qPCR was completed with 40 amplification cycles as follows: denaturation at 95 C for 15s, annealing at 58 C for 30s, and extension at 72 C for 42s

The Thermo UltiMate 3000 high-performance liquid chromatograph (United States) was utilized to achieve chromatographic separation of the target compounds using a Thermo Accucore C18 column (100 mm 2.1 mm, 2.6 m) (United States)

Epilepsy is a neurological and a systemic disorder

Before the IV injection, I didnt have the desire to go out or do much of anything