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Comprehensive toxicology evaluations reveal that the compound does not affect food intake, body temperature regulation, or basic physiological parameters at effective doses in animal models, indicating that its metabolic effects occur through specific pathway modulation rather than general physiological disruption

One study investigating these effects in obese mice discovered that administration of an NNMT blocker reduced insulin serum levels by 50-60% in the subjects

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However, the convenience of a single morning dose means most researchers prefer once-daily administration, and the clinical data supporting split dosing specifically for AOD 9604 is limited