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After rinsing with PBS, all sections were incubated at room temperature for 2 h with IgG goat anti-rabbit (Thermo Fisher Scientific, Alexa Fluor 488) diluted 1:200 in 0.01 M PB containing 1% BSA and 0.3% Triton-X100

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Equal amounts of the protein were separated on an SDSpolyacrylamide gel

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(143) elucidated the adverse effects of oxidative stress and chronic inflammation on ovarian functionality, suggesting that exogenous estrogen supplementation may mitigate chronic low-grade inflammation and enhance follicular development