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Calcium influx was measured using flow cytometry for 5 min on a BD FACSymphony A5 flow cytometer, and calcium flux was quantified as the ratio of Indo-1 emission at 400 nm (bound Ca 2+ ) to 475 nm (free Ca 2+ )

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Mass spectrometry approaches can also be coupled to isotope tracing to gain insights into flux, for example, using [1,2- 13 C]glucose and searching by imaging mass spectrometry for glycolytic intermediates with one 13 C atom to capture oxPPP overflow flux at the single-cell level