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Immunofluorescence staining Ovarian tissues and GCs were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100, followed by incubation with the corresponding antibodies at 4 C overnight

Rheum officinale enema can also reduce TMAO and TMA levels in the serum of 5/6Nx CKD rats by decreasing certain TMAO-related bacteria, inhibit the expression of inflammatory markers (interleukin-6, tumour necrosis factor-, and interferon-), alleviate renal interstitial fibrosis, and slow the progression of CKD (Ji et al., 2021)

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Here, co-exposure to both Cd and HgCl 2 for 28 days leads to further collagen deposition (Arbi et al