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Description

Why and how to investigate the role of protein phosphorylation in ZIP and ZnT zinc transporter activity and regulation

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To synthesize cDNA, 500 ng of RNA was used for reverse transcription using iScript Reverse Transcription Supermix (Bio-Rad, 1708841) according to the manufacturers instructions with the thermal cycle as follows: 5 minutes at 25 C, 20 minutes at 46 C and 1 minute at 95 C

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