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"We urge our members to follow their recommendations, and hope that we will attain our goal of bringing the prevalence, the age of onset, and the severity of the disease to the levels seen in other breeds of dogs." The Club also mailed to its members annually, beginning in 1998, a Health Registry booklet, which included this set of MVD breeding guidelines: Even with these strong endorsements, only a handful of cavalier breeders have faithfully followed the MVD breeding protocol

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For normalization among the samples, at least 20 core metabolites (amino acids and nucleotides) were quantified from each sample in negative and positive mode with El-MAVEN (0.12.0) 45