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Excess probes were washed off with 2x saline sodium citrate (SSC) (Cat#15557-044, Invitrogen) and deionised formamide (Cat#AM9342, Ambion), followed by a 30 min incubation with buffer W (#121300313, Nanostring slide preparation kit) to block nonspecific signal

Patients have unique microbiome fingerprints, so future efforts must focus on personalized microbiome medicine

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