purification of proteins fused to glutathione s transferase GST-Tag What is a GST Pull-Down

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Metformin has been used as a medication since the 1950s and has become one of the most commonly prescribed medications for managing diabetes and other conditions like PCOS and prediabetes

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To permeabilize the cuticle, the worms were incubated for 1 h at room temperature with gentle rocking in 1 Modified Ruvkuns Witches Brew buffer (diluted from a 2 stock: 3.7% formaldehyde, 160 mM KCl, 40 mM NaCl, 14 mM EDTA, 0.2% ME)