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For cell cycle analysis 10 6 cells/condition were fixed and permeabilized with cold 70% ethanol

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47 The TNFR1-RIP-TRADD-TRAF2 complex plays a crucial role in regulating cell survival and apoptosis 48 ,49 , and when TNFR1-mediated signaling successfully activates the complex and NF-B signaling, the cells will survive in the presence of FLICE inhibitory proteins (FLIP, Caspase-8 inhibitor), and conversely lead to cell death

F.NardinP.et al (2011)

Iron overload promotes mitochondrial fragmentation in mesenchymal stromal cells from myelodysplastic syndrome patients through activation of the AMPK/MFF/Drp1 pathway