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In contrast, TIGAR overexpression or treatment with Fer-1, a ferroptosis inhibitor, significantly increases NADPH, GPX4, and GSH levels, reduces GSSG expression, and attenuates neuronal ferroptosis and neurological impairment

Bone turnover markers: In the elderly control group, OC decreased by 45% and CTX increased by 75%

Vitamin K protects tissues from ferroptosis We then tested whether vitamin K can also prevent ferroptosis in vivo using mice with genetic deletion of Gpx4 and pathological models, in which ferroptosis results in tissue injury

Cell cycle analysis The position of the cells in the cell cycle was determined by flow cytometric analysis of the DNA content using propidium iodide

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