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EdU click reaction was performed in buffer containing 100 mM Tris-HCl (pH 8.5), 2 mM CuSO 4 , 100 mM sodium ascorbate, and 5 M Alexa Fluor 488 Azide (Thermo Fisher Scientific, A10266) for 30 min in the dark followed by two washes with 1% (w/v) BSA in PBS

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maintenance = 2.25 mg weekly - closely matching the standard protocol

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