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Description

Tissue factor expression by endothelial cells in sickle cell anemia

The gut microbiota is a diverse system that plays major roles in the healing of tissues, metabolism of nutrients, and protection against infections (Thursby and Juge, 2017)

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10.2174/1389201023666220520102001 60 LiX.LiB.ShiY.WangC.YeL

In brief, RNA was extracted from cells of the indicated genotype using the Qiagen RNAeasy RNA extraction kit (Qiagen, Manchester, UK) following the manufacturers instructions.mRNA (1 g) was retrotranscribed into cDNA using High Capacity RNA-to-cDNA kit (Life Technologies, Paisley, UK)