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Compared to conventional imaging probes in the visible region, fluorescence imaging in the NIR-II region (10001700 nm) exhibits deeper tissue penetration and higher spatiotemporal resolution due to reduced tissue autofluorescence and photon scatter, enhancing accuracy and reliability in disease detection [13], [14], [15], [16], [17], [18]

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Cell lysates were subjected to ultrasonic disruption on ice for 5 min, followed by centrifugation at 15,000 g for 10 min at 4 C to remove insoluble debris

Determine the concentration of GSH in the sample solution using a calibration curve

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