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Approval for the study protocols was granted by the Ethics Committee of Fujian Maternity and Child Health Hospital (Reference: 2021 KLRD09007)

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Inflammation Bowel Dis (2011) 17:787

The BUTE-CM supernatant was extracted and stored in 80 C until future ultracentrifugation or subject to immediate ultracentrifugation using a Beckman Coulter L-80 Optima Ultracentrifuge at 4 C

The ensuing ligation brought out a correctly assembled plasmid, which could be verified by restriction digest as well as sequencing (Fig