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The reactions were performed in a 25 l volume of KAPA SYBR FAST qPCR kit (Kapa biosystems, USA) with 10 picomole of forward and reverse primer from each gene ( Table 1 ) using Mx-3000P spectro flourometric thermocycler (Stratagene, USA)

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New confusion, weakness, poor oral intake, breathlessness, or reduced urine output in an older adult should be taken seriously regardless of temperature