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A reduction in IFN- and IL-2 release from T cells was detected when T cells were co-stimulated with anti-CD3 and anti-CD28 using the Leu analog N-acetyl-Leu amide (NALA)

concentrations greater than 60 M were not tested due to known cytotoxic effects in 3T3-L1 cells

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The second approach consists in technologies, such as differential display, DNA microarrays or Proteomics, able to simultaneously measure modifications of a very large number of genes/gene products

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