glutathione pkas and glutathione disulfide – their biomedical and pharmaceutical applications | Medicinal Chemistry Research Glutathione-Related Enzymes and Proteins: A

Zou Y, Palte MJ, Deik AA, Li H, Eaton JK, Wang W, Tseng YY, Deasy R, Kost-Alimova M, Dancik V, Leshchiner ES, Viswanathan VS, Signoretti S, Choueiri TK, Boehm JS, Wagner BK, Doench JG, Clish CB, Clemons PA, Schreiber SL (2019) A GPX4-dependent cancer cell state underlies the clear-cell morphology and confers sensitivity to ferroptosis

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The soluble fraction was clarified by centrifugation (75,000 g , 20 min, 4 C) and incubated with 50% Ni-Sepharose suspension (GE Healthcare, Chalfont St Giles, UK) for 2 h at 4 C with gentle rotation prior to affinity purification

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Two hydrogel samples were integrated without supplementary stimuli