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putida KT2440 showed there might be a different -1 linkage breakdown mechanism compared to Gram-negative bacteria Novosphingobium aromaticivorans DSM12444 [41]

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Cells were maintained in 75 cm 2 flasks (Corning Inc., Corning, NY, USA) at 37 C in a 5% CO 2 humidified atmosphere in Dulbeccos modified eagle essential minimum medium (DMEM) (Gibco, ThermoFisher Scientific, Life Technologies, Waltham, MA, USA) supplemented with 15% inactivated fetal bovine serum (FBS) (Wisent In., St-Bruno, QC, Canada), 0.1 mM non-essential amino acids, and penicillinstreptomycin (50 U/ml to 50 g/ml) (Gibco Life Technologies), pH 7.3