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Clonogenic assay For long-term assessment of drug treatment, cells (2 10 3 ) were seeded in 6-well plates and incubated overnight

Nat Med (2022) 28:233343

In the context of ICB, therapeutic efficacy relies on reinvigorating endogenous T cells by blocking inhibitory receptors at the cell surface

Based on these findings, a randomized phase II clinical trial compared the efficacy and safety of MK-2206 to the mTOR inhibitor Everolimus in patients with advanced RCC resistant to VEGF targeted therapy ( Table 2 ) (227)

These off-target mechanisms also include improvement in hematocrit, blood pressure and body weight which can all contribute to positive renal and heart effects (130134)