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Endocrine Research

LEfSe is known to reduce false positives and can highlight biological consistency across subclasses, while the KruskalWallis test used alone can achieve a lower false-negative rate compared to LEfSe 38

Riboflavin analysis Sample preparation Riboflavin was extracted with 5 volumes of a mixture of methanol/acetonitrile/water (2/3/1) and 10 L internal standard added

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