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Electroacupuncture may inhibit oxidative stress of premature ovarian failure mice by regulating intestinal microbiota

For the cytoplasm and muscle, the sections were then stained in Biebrich scarlet-acid fuchsin solution for 1015 min, again washed with distilled water and treated with phosphomolybdic-phosphotungstic acid solution for 1015 min, at this step, and for collagen staining, slides were transferred to aniline blue solution for 510 min and rinsed with water and 1% acetic acid

Factors such as skin type, genetics, dosage, and the quality of injections can all influence the outcomes

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For the evaluation of cell survival, mice were divided into four groups of young and aged mice, respectively: (1) sham-operated WT mice (youngWTsham, n = 8