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Cortisol is widely recognized as a key indicator of stress in fish, with levels rising in response to stressors 23,90,92

Western Blot Analysis RIPA lysis buffer and Bradford Protein Assay Kit were used for protein determination from brain tissues (Bio Basic Inc., Markham, Ontario, Canada)

Model robustness was assessed using Receiver Operating Characteristic - Area Under Curve (ROC-AUC) analysis in MetaboAnalyst software

MI is a frequently employed and extensively researched therapeutic approach that can be used as a brief, stand-alone intervention or alongside other pharmacologic or nonpharmacologic psychotherapeutic options

Fungi, bacteria, viruses, and bacteria are all considered microbiomes