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doi: 10.1007/978-3-319-66653-2_7

The protocol comprised eight stressors: food deprivation for 24 h, water deprivation for 24 h, flash stimulation (150 flashes/min for 5 min), cage tilting at 45 for 24 h, overnight illumination for 8 h, wet cage environment (200 mL water added to sawdust bedding) for 24 h, tail suspension for 5 min, 60 C heat stimulation for 6 min, and nipped tail for 5 min

Yet, increased spatially localized ROS levels can also promote cell toxicity thereby leading to the activation of cell cycle arrest or cell death-inducing pathways resulting in the inhibition of cancer progression [108, 109]

Many individuals notice reduced hunger and increased fullness within the first one to two weeks, while noticeable weight loss often takes several weeks longer

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