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two cohorts received disulfiram 250 mg and 500 mg daily, respectively (Schweizer et al., 2013)

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Protein signals were developed with SuperSignalTM West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, USA) and imaged using a chemiluminescence imaging system MiniChemi 610 with SageCapture TM software version 2.17.12.170316 (Sagecreation, China)

Interestingly, although lipid peroxidation amplifies PARP-1 activation, inhibiting PARP-1 can significantly reduce lipid peroxidation and maintain mitochondrial integrity, underscoring the link between PARP-1 overactivation and oxidative damage in promoting parthanatos [142, 143]