Boosting NAD + levels through the PreissHandler pathway is achievable through NA supplementation 28,29
The international journal of biochemistry & cell biology, 33(3), 205220
Investigation: R.G., K.J., P.A.G., F.L., M.N., J.H., A.W., S.B., F.P., M.D., N.K., V.K., C.C., V.N.M., N.L.C., E.S.H., H.L., K.W., D.I., T.P.W., C.S., M.J., M.A.C., F.A.M., E.T.K., I.T., B.H.C., F.O., E.F.F
Standard reconstitution (10mg/ml) Most common protocol: 50mg GHK-Cu powder Add 5ml bacteriostatic water Creates 10mg/ml concentration Easy math for dosing How to calculate doses: 1mg dose = 0.1ml (10 units on insulin syringe) 2mg dose = 0.2ml (20 units) 3mg dose = 0.3ml (30 units) Total doses: 25 doses at 2mg each Why this concentration works: Simple calculations Easy to measure Standard for anti-aging protocols Fits insulin syringe well Alternative reconstitution (5mg/ml) More dilute option: 50mg GHK-Cu powder Add 10ml bacteriostatic water Creates 5mg/ml concentration Dose calculations: 1mg dose = 0.2ml (20 units) 2mg dose = 0.4ml (40 units) 3mg dose = 0.6ml (60 units) Total doses: 25 doses at 2mg each Why use 5mg/ml: Larger injection volume (some prefer) Easier to measure precisely Less concentrated (gentler on tissue) Good for beginners High concentration option (20mg/ml) Concentrated protocol: 50mg GHK-Cu powder Add 2.5ml bacteriostatic water Creates 20mg/ml concentration Dose calculations: 2mg dose = 0.1ml (10 units) 3mg dose = 0.15ml (15 units) 4mg dose = 0.2ml (20 units) When to use: Want smaller injection volume Using higher doses (3-5mg) Experienced users Prefer concentrated solutions Recommendation: Start with 10mg/ml (50mg + 5ml water) - simplest and most versatile
doi:10.1016/0024-3205(94)00796-9 30
