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Conclusions Ultimately, the current maintenance dose of 1,000 mcg B12 every 3 months may confer long term harm, therefore it seems that once B12 levels are stabilised well within acceptable range, then the standard B12 intervention of 1000 mcg should be administered annually, and monitored with pre-injection annual testing of levels

Detection windows are not publicly established with precision, but peptide metabolites can persist for days to weeks depending on testing sensitivity

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This article examines the clinical implications of B12's water solubility for absorption, metabolism, and supplementation strategies