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The vitality of cells in each well was assessed as a reference value after four h of seeding

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Considering this, detected the expression levels of autophagy markers (LC3B and PINK1), antioxidative stress markers (SOD1 and GPx1), mitochondrial uncoupling proteins (UCP2, UCP4, and UCP5), and mitochondrial factors including pro-mitochondrial fission factors of dynamin-related protein 1 (DRP1) and its phosphorylated version of p-DRP1, the mitochondrial fission factor (MFF) in charge of pro-midzone-type fission, the mitochondrial fission 1 (FIS1) in charge of pro-peripheral-type fission, and pro-mitochondrial fusion factor mitofusin 2 (MFN2), in SNr and cerebral cortex)

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When inhaled through nebulization, NAC is absorbed directly into the lungs, where it helps break down mucus, neutralize toxins, and promote tissue repair