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Non-esterified short-chain fatty acid (SCFA) quantification After finding that nonesterified short-chain fatty acids (SCFAs) were not detected by our first approach, we measured acetic, propanoic, isobutanoic (isobutyric), butanoic (butyric), isopentanoic (isovaleric), pentanoic (valeric), hexanoic (caproic) and heptanoic acids with a Clarus 500 chromatography system connected to a SQ8s mass spectrometer (Perkin Elmer) using the method described by Togo et al 50

18 Sequence analysis Amino acid sequences were selected from NCBI and UniProtKB databases: Homo sapiens (code: P09211), Bos taurus (code: NP_803482.1), Capra hircus (code: Q9TTY8.2), Equus caballus (code: XP_001498156.1), Ovis aries (code: XP_004019771.1) and Sus scrofa (code: M3V836)

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One week post-surgery, rats received intraperitoneal injections of IR-780 (0.667 mg/kg) or an equivalent volume of PBS solution twice weekly for 3 weeks

This study demonstrated that, upon fasting, SREBP1c protein in the liver is rapidly degraded by E3 ubiquitin ligase ring finger protein 20 to suppress unnecessary lipogenesis