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Although we currently do not know the time required for vesicle reuse, recent studies using hippocampal synapses as well as cerebellar mossy fiber terminals in slices (Watanabe et al., A suggestion of rapid reuse of incompletely filled vesicles comes from experiments in which vesicular glutamate transport was abolished by dialyzing presynaptic terminals with a glutamate-free pipet solution, while EPSCs were continuously monitored (Hori and Takahashi, + buildup of endocytosed vesicles with strong buffers, results in rapid synaptic depression in hippocampal preparations (Ertunc et al., A second indication comes from an experiment in which the input-output relationship of presynaptic and postsynaptic AP firings is monitored, while vesicular glutamate transport is slowed by changing the presynaptic Cl concentrations (Nakakubo et al., Figure 5A)

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Segain JP, Raingeard de la Bletiere D, et al

The Immediate Effect of COVID-19 vaccination on Anticoagulation Control in patients using vitamin K antagonists