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The membranes were blocked with 5 % skim milk (dilution in TBS with 0.05 % Tween 20 (TBST)) and incubated with primary anti-bodies (1:1000, dilution in TBST) for overnight at 4C

Cell Host Microbe (2017) 22(4):51930 e3

However, chronic, pathological levels of non-radical ROS in the tumor microenvironment conversely fostered immunosuppression and therapy resistance, highlighting their starkly context-dependent impact [3, 241]

To separate a phenotype caused by Gpx4 -deficiency from that of Cre side effects, tamoxifen-treated mice harboring Gpx4 fl/fl ;Cre ERT2 and Gpx4 wt/wt ;Cre ERT2 BM cells were allowed to recover and blood was drawn every three weeks (Figure 1)

DNA damage and oxidant stress activate p53 through differential upstream signaling pathways