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Relative expression was calculated using the 2 -Ct method, with GAPDH as an internal control

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Treatment of the second aliquot with TCEP and IAM, followed by measurement of CAM-S-CAM-TPP after protein precipitation, gave the total amount of RSS /RS(S) n S present in the tissue homogenate, giving values that are consistent with earlier reports of overall tissue levels of persulfides using sulfane sulfur atom trapping 31,32

Gently swirl/roll until dissolved (do not shake) [8]

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