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Protein expression was induced by 0.2 mM IPTG at 18 C overnight in TB media supplemented with ampicillin (100 g/ml), kanamycin (25 g/ml) and spectinomycin (50 g/ml)

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Free Radic Biol Med 191:150163 Rueli RH, Torres DJ, Dewing AS, Kiyohara AC, Barayuga SM, Bellinger MT, Uyehara-Lock JH, White LR, Moreira PI, Berry MJ et al (2017) Selenoprotein S reduces endoplasmic reticulum stress-induced phosphorylation of tau: potential role in selenate mitigation of tau pathology

Cells were imaged in fresh FluoroBrite DMEM supplemented with 4 mM glutamine, 1 mM sodium pyruvate, 10% FBS and penicillin (100 U ml 1 )/streptomycin (100 g ml 1 )/fungizone (0.25 g ml 1 )

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