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People with symptoms of vitamin B12 deficiency It is a good idea for anyone with the following signs and symptoms of a vitamin B12 deficiency or pernicious anemia to consult a doctor: People with gastrointestinal issues Conditions that affect the gastrointestinal tract may affect vitamin B12 release or absorption, including: pernicious anemia, which can lead to gastric atrophy, or damage to the stomach fish tapeworm infestation bowel or pancreatic cancer folic acid deficiency overgrowth of bacteria in the small intestine diabetes thyroid disease Crohns disease celiac disease chronic pancreatitis People who have undergone gastrointestinal surgery, including weight loss, may have fewer cells necessary to secrete stomach acid and intrinsic factor

Monocyte isolation and cell culture Human peripheral blood mononuclear cells (PBMCs) were isolated from the obtained leukopaks using density gradient centrifugation with Ficoll-Paque Plus (Cytiva, Marlborough, MA)

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At 10 mg/mL, accuracy becomes critical

Non-alcoholic fatty liver disease (NAFLD) affects a majority of individuals with obesity