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Dysbiosis, an imbalance in the gut microbial community, is frequently observed in individuals with ASD and is associated with alterations in key neurotransmitters, such as GABA, serotonin (5-HT), and DA, which are crucial for regulating mood, cognition, and social behaviors [179]

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Cells were maintained in a humidified environment at 37C under a 5% CO2 atmosphere for 48 h

Research cycles typically run for 4 to 8 weeks, often followed by a break of 2 to 4 weeks before potentially resuming