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Both: monitor hormonal panels, inflammatory markers, and reproductive imaging

Significant Unknowns Because no human-approved protocols exist, major uncertainties remain: whether localized vs systemic models produce different outcomes how timing affects various tissue types how combined administration influences biological pathways long-term safety implications of repeated dosing optimal duration, interval, or amount for any experimental purpose These unknowns highlight the experimental nature of all administration approaches described in preclinical settings

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Across GLP-1 trials, roughly 60% of lost weight returns within a year and about 75% over the longer regain curve. Lower-dose maintenance has stronger evidence than complete withdrawal