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[0059] Active agent or active ingredient, whether singular or plural, refers to an ingredient(s) included in the toothpaste to address, support, or sustain some aspect of the health, well-being, and/or appearance of the skin and/or is added for a specific purpose other than dental hygiene and to exert some influence on the user when used, and particularly when swallowed and/or absorbed in or from the oral cavity

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The primers for PCSK6 were designed and purchased from Sangon biotech (Shanghai, China): PCSK6 (forward, 5-ACTCCAGAAGAAGAGGAAGAGTA-3

This mathematical method must meet two assumptions: reaching the maximum response and recording at least two points located near the targeted point of the 50% maximal response

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