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The supernatant was transferred to a new tube

Cell culture and drug treatment The human liver cancer cell line HepG2 was obtained from ATCC and was cultured in DMEM glutamax high glucose medium (Invitrogen) supplemented with 10% fetal bovine serum and 100 U/mL penicillin/streptomycin (Invitrogen), in a humidified incubator at 37C and 5% CO 2 atmosphere

Haemoglobin-bound iron is transported into the intestinal epithelial cells by heme carrier protein 1 (HCP1) that is localised to the intestinal mucosal epithelium

In 2 women who used them very frequently (up to 3x-4x/hour), coffee enemas caused severe electrolyte imbalances that resulted in death [77+]