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The radioactive proteins synthesized are separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and detected by radioautography

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Meanwhile, most other antioxidants are either water- or fat-soluble ( For instance, vitamin C is only water-soluble, while vitamin E is only fat-soluble

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Alternatively, for immunoprecipitation or viral production, cells were transfected with polyethylenimine (PEI) (Sigma-Aldrich) in 3:1 PEI:DNA weight ratio