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The antioxidant role of GSH GSH is implicated in several functions, including antioxidant defense with reduction of oxidative stress and maintenance of redox balance, metabolic detoxification from xenobiotics and exogenous compounds, cell cycle regulation, and immune system modulation, as well as fibrogenesis ( Its main role is to shield cellular macromolecules from endogenous and exogenous reactive oxygen species (ROS) and nitrogen ones

The stable luminescence is produced by the conversion of Luciferin-NT, a GSH probe, to luciferin by a glutathione S-transferase enzyme

However, for the same concentration range, a constant exposure can yield a harmful depletion of GSH to 30% of normal after 14 hours, indicated by the black-dashed line (Fig

This assay is a convenient and sensitive screening methodology to detect and measure both inhibitors and activators of sirtuin activity

doi: 10.1136/gutjnl-2021-324243 63