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Noticeable induction of MT3 in the roots was observed with slight upregulation of GS2 and PCS1 at late period of Hg exposure (Figure 5), suggesting that MT3 is the crucial gene that confers diminished Hg toxicity, with the cooperation of GS2 and PCS1 occurring at the late stage of Hg stress

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Some researchers also track NAD+ metabolite levels via urinary nicotinamide excretion to confirm functional NNMT inhibition, though this is not standard practice

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