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Enzymatic assays To assay the activities of Cp1A and MfaA, 100-l reactions were performed at 30 C for 3 h in 50 mM sodium phosphate buffer (pH 8.0) containing 0.2 mM FeSO 4 , 2 mM KG, 2 mM ascorbate, 1 mM substrate and 10 M Cp1A or MfaA

En particular, la magnitud de la reduccin de GSH parece ser paralela a la gravedad de la enfermedad

Secondly, HOCl/OH can activate enzyme complexes involved in DNA repair (such as APE1, PARP, DNA ligase) to assemble at DNA damage sites, promoting chromatin decondensation and further enhancing NETs/NETosis [247]

Este equilibrio redox regula diversos procesos metablicos intracelulares que incluyen la actividad enzimtica, el transporte celular, transduccin de seales y la expresin gnica mediada por la transcripcin de factores entre los que se destacan el activador de protena (AP-1), el factor nuclear kappa-p ( NF k-B) y el p53 (4)(26)(33)

S1 ), the mesh insert was slowly transferred into liquid nitrogen at 196 C, and subsequently transferred into a 150 C freezer for long-term storage (up to 7 days)