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Cells in the HG condition were cultured in the medium containing 35 mM of D-glucose (Sigma-Aldrich), and cells in the SG condition were cultured in DMEM/F12 (this represents control, CTR, condition)

Together, they address two sides of a body transformation that fat-loss-only protocols often leave incomplete: the metabolic engine that drives fat loss, and the tissue quality that determines how that fat loss looks and feels in practice