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It activates lipolysis, the breakdown of stored fat, while inhibiting the formation of new fat

Funding This work was supported by Heilongjiang Provincial Natural Science Foundation of China (LH2021C061)

Protein purification XPO1 (also known as CRM1) was purified as described 109 with a minor modification: 4 L of LB medium were used instead of 6 L

However, under oxidative stress, Bach1 dissociates from the HO-1 promoter, and stress signals induce the dissociation of Nrf2 from Kelch-like ECH-associated protein 1 (Keap1), which then binds to AREs and initiates the downstream expression of oxidative stress-resistant HO-1 to prevent ROS-induced cellular damage and maintain the organisms antioxidant response (Zhang et al., 2019)

Furthermore, to prevent systematic bias from the analytical sequence, all samples were randomized and analyzed with an inter-dispersed QC strategy, whereby LQC, MQC, and HQC samples were injected after every 9 clinical samples, establishing a closed-loop quality control system to ensure data accuracy