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GLUD1 has two co-enzyme binding sites: one in the NAD-BD that is able to bind ether NAD+ or NADP + and is directly involved in the catalytic process, and a second one, that has regulatory function, lying directly under the pivot helix, that can bind ADP, NAD + , or NADH, but does not bind NADPH well

(Pro tip: Take this strategy even further by adding a third antioxidant all-star, N-acetyl-L-cysteine or NAC for short, to this lineup!) Can I Take Too Much Vitamin C

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To confirm the use of the Hill equation, K D s calculated by fitting to the quadratic solution (Supplementary Table 1) were found to be within experimental error of K D s calculated by the Hill equation (for encapsulated samples, an RNA concentration of 58 nM was used for the fit to the quadratic solution)

CD analysis of an extracted G-rich region (126 nt) confirmed K-dependent G4 folding (Fig