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In summary, our results mostly align with previous research in that some of the common KEGG pathways are involved during IC/BPS pathogenesis

The cells were incubated at 37 C in 5% CO 2 overnight

Pharmacol Ther 138(2):176184 Karasek M, Reiter RJ (2002) Melatonin and aging

Small-bowel mucosal changes and antibody responses after low- and moderate-dose gluten challenge in celiac disease

The study lasted 9 weeks, during which clinical signs associated with biotin deficiency and biochemical parameters, such as serum and tissue biotin levels (liver, kidney, brain, testes), and biotin-dependent enzyme activity of 3-methylcrotonyl CoA carboxylase (MCC), propionyl-CoA carboxylase (PCC), pyruvate carboxylase (PC) and acetyl-CoA carboxylase (ACC), and blood glucose levels, were evaluated