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Colony formation and tumor spheroid formation assay For colony formation assays, 2000 cells were placed into each well of 6-cm dishes and cultured for 2 weeks

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Oxamate, a pyruvate analog, competes for the active site of LDH, displaying antitumor activity in diverse cancer cell lines, such as gastric, medulloblastoma, cervical, liver, and non-small cell lung cancers

Treatment Protocol Dosage 1-2 mg per injection, individualized by your physician based on clinical indication, IGF-1 response, and tolerance Frequency Once daily subcutaneous injection, typically in the evening to align with the physiological overnight GH pulse Duration 12-26 weeks per cycle, reassessed at each clinical review based on IGF-1 levels, visceral fat response, and patient goals Administration Subcutaneous injection under physician supervision with a prescribed dose and monitoring schedule