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Morsy, A., El-Zaiat, H., Saber, A., Anwer, M., & Sallam, S

Phylum-level analysis (Figure S1D) revealed that the VPA-ASD group was dominated by Pseudomonadota (Proteobacteria), with elevated Deferribacterota and Campylobacterota, and severe depletion of Bacteroidota and Verrucomicrobiota

MMNST often carry an inactivating mutation in the protein kinase A inhibitory subunit gene protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A) on chromosome 17p22-24, which acts as a tumor suppressor [3, 4]

As these points were only present in PLA and were not influential in the overall outcomes, all data points were retained in the analysis

Cells were treated with either glutathione diethyl ester (GDE) to enhance intracellular GSH or L-buthionine sulfoximine (L-BSO) to inhibit GSH synthesis